On 29 January 1992 UN Security Council unanimously accepted in its session a resolution with regard to the General Assembly's recommendation to accept the Republic of Tajikistan to the membership of the Organization. On 2 March 1992 in 46th Session of UN GA the Resolution 46/224 unanimously was adopted with regard to acceptance of the Republic of Tajikistan to the UN.  The country's joining to Nations Community laid fundamental basis for development of its cooperation with the specialized organizations and institutions of UN.

UN Representation in Dushanbe was opened on 1 February 1993. The activities of UN Mission of Observers to Tajikistan (21 January 1993 - May 2000) through assistance to peacemaking efforts and assessment of  war situation in the country secured progress of  peace process in Tajikistan, rendered assistance in providing urgent humanitarian aid.

As a result of implementation of the General Agreement in May 2000 UN Tajikistan Office of Peace-Building was set up upon the proposal of UN General Secretary Koffi Annan.  

The Representation of UN Development Programme was established in December 1993. The same year the Country Agreement between the Government of the Republic of Tajikistan and UNDP. was signed. The Agreement laid legal basis for the Organization activities  in the country. The framework agreement on cooperation between the Government of the Republic of Tajikistan and UNDP initially was approved for the period of 1999-2000, then was extended till 2003. The Agreement includes such a fields as state government  and creating potential in local level; reconstruction and development; protection and management of natural resources. In March 2003 the framework agreement was extended till the end of 2004. After the General Agreement on Peace and National Reconciliation in Tajikistan was signed in 1997 UNDP has reviewed its main projects and programmes. Upon the recommendation of the Government of Republic of Tajikistan the emphasis was  placed on long-term projects and programmes oriented to the sustainable development and reconstruction of national economy of Tajikistan.

For the purpose of all-round development of international cooperation in the field of combating illegal turnover of drugs Tajikistan set up sound legal basis both within the framework of UN and with region's states. In 1996 Tajikistan signed the Memorandum on cooperation of Central Asian Countries in the field of control on illegal production, turnover, abuse of drugs, psychotropic agents and precursors. Tajikistan has joined the United Convention on drugs of 1991, the Convention on psychotropic agents of 1971, the Convention on combating illegal turnover of drugs and psychotropic agents of 1988 and the Convention on psychotropic agents.

On 27 April 1999 the President of the Republic of Tajikistan and the Director General of UN Office on Drugs and Crime signed Protocol with regard to organizing the Agency of control on Drugs on the basis of existing state commission. Its functions consists in realization of laws on combating illegal turnover of drugs, preventing and treatment of drug addiction as well as investigation of crimes related to narcotics. Since June 1999 representation of UN Regional Office on drugs and crime has been operating in Tajikistan.    

UN High Commissioner for Refugees Office started to operate in Tajikistan in January 1993. The initial main object of UNHCR was assistance to voluntary repatriation of the Tajik refugees from Afghanistan and CIS countries, as well as to help them in the process of rehabilitation and integration through involving them in participation in different projects and programmes. At present mandate of UNHCR Office in Tajikistan is confined to supporting the Afghan refugees in the country.

UN Children Fund (UNICEF) began to work in Tajikistan in May 1993, shortly after the independence of the Republic. UNICEF Tajikistan is mandated to share its rich global experiences in the area of children and women's rights realization with the countries in which it has offices based on their national and local situation analysis and priorities within the framework of international commitments for children and women.

World Food Programme Office was opened in Tajikistan in May 1993. The main mission of the Office is providing humanitarian food aid to refugees and displaced persons.

World Health Organization opened its office in Dushanbe in 1993. The Organization's activities are mainly directed at control on infectious diseases, psychological and physical rehabilitation of persons suffered from war, as well as support to national health system of the country.

Food and Agriculture Organization Office in Tajikistan was opened in May 1997. The main direction of FAO's activities is assistance to the development of agricultural sector of the country.

International Monetary Fund opened its representation in Tajikistan in 1997. IMF renders assistance in providing mechanism for consultation and coordinated efforts with regard to international monetary issues, assists to the balanced growth of international trade share in economy of the Republic of Tajikistan.

World Bank began its work in Tajikistan in 1996. Its activities are directed at rendering technical assistance and providing loans for the support of national currency and country's economy.

UN Development Fund for Women (UNIFEM) has been working in Tajikistan since 1999. Its activities are directed at realization of several special projects aimed at providing additional support to programmes and strategies of the Government of Tajikistan regarding women rights protection and widening their participation in society's life.